Electrophoretic identification of whitebait species
نویسندگان
چکیده
The shortjawed (SJ) kokopu (Galaxias postvectis ) is rare and listed as category A conservation status by DOC and as indeterminate (status not resolved) by IUCN. It may be in decline given that there is substantial habitat loss and modification. If existence of SJ kokopu is to be maintained, information regarding its presence within whitebait runs both in terms of timing and geography, is essential. Both SJ kokopu and koaro (Galaxias brevipinnis) are involved in the whitebait fishery and commonly occur together with three other Galaxias spp. Unfortunately, morphological data do not allow the discrimination between koaro and SJ kokopu whitebait, making it impossible to study the ecology of SJ kokopu whitebait independently. Using information encoded within the DNA we developed genetic markers from the easily identified adult fish for G. brevipinnis, G. postvectis, G. maculatus and G. fasciatus . Using these markers we screened 48 whitebait morphologically identified as being either G. brevipinnis or G. postvectis . Of these 48 individuals, 42 were shown to be G. brevipinnis, two were shown to be G. fasciatus and four individuals produced no genetic markers (i.e. the DNA was degraded). None were shown to be G. postvectis . Because none of the whitebait screened were SJ kokopu, we were unable to proceed to identification of distinguishing morphological features. Results have also shown that the traditional method of identification of species using morphological features can be inaccurate.
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